Lab Publications

RNA, Biochemistry, eLife, Nucleic Acids Res, Nat Commun, Cell Chem Biol, Methods, J Biol Chem, Nucleic Acids Res., Proc. Natl. Acad. Sci. U.S.A., Wiley Interdisciplinary Reviews RNA, Proc Natl Acad Sci USA, J Mol Biol., ChemBioChem, Journal of Molecular Biology, PLoS One, ACS Med Chem Lett, Chem Soc Rev, Chemical Communications (Cambridge, England), Mol Cell, Science, mSphere, Biophysical J, Structure, Curr Opin Chem Biol, Methods Enzymol., Wiley Interdiscip Rev RNA, Analyst, J. Mol. Biol., Encyclopedia of Cell Biology, Curre Biol., Trends Biochem. Sci., RNA Biology , Cell Rep, Chem Commun (Cambr)., Nat Protoc, Crit Rev Biochem Mol Biol,

Liu Y, Paulson JC, and Hoskins AA. 2025. Control of 3' splice site selection in S. cerevisiae by a highly conserved amino acid within the Prp8 α-finger domain RNA 32(1):82-96 PMC12707134
Love SL, McKeon MC, Vollmer H, Paulson JC, Oshimura N, Valentine O, Ortiz SC, Hull CM, and Hoskins AA. 2025. The pre-mRNA splicing modulator pladienolide B inhibits Cryptococcus neoformans germination and growth mSphere 10(7):e0024825 PMC12306170
Liu Y, Nomura Y, Butcher SE, and Hoskins AA. 2025. RNA modifications and Prp24 coordinate Lsm2-8 binding dynamics during S. cerevisiae U6 snRNP assembly J Biol Chem 301(5):108497 PMC12136795
Fu X and Hoskins AA. 2025. Dynamics and evolutionary conservation of B complex protein recruitment during spliceosome activation Nucleic Acids Res 53(4):gkaf124 PMC11850228
Carrocci TJ, DeMario S, He K, Zeps NJ, Harkner CT, Chanfreau GF, and Hoskins AA. 2024. Functional analysis of the zinc finger modules of the Saccharomyces cerevisiae splicing factor Luc7 RNA 30(8):1058-1069 PMC11251517
Senn KA and Hoskins AA. 2024. Mechanisms and regulation of spliceosome-mediated pre-mRNA splicing in Saccharomyces cerevisiae Wiley Interdiscip Rev RNA 15(4):e1866
Beard JP, Love SL, Schmitz JC, Hoskins AA, and Koide K. 2024. Structure-Activity Relationship Study of Splicing Modulators on Hsh155/SF3B1 through Chemical Synthesis and Yeast Genetics ACS Med Chem Lett 15(12):2225-2230 PMC11647714
Senn KA, Lipinski KA, Zeps NJ, Griffin AF, Wilkinson ME, and Hoskins AA. 2024. Control of 3' splice site selection by the yeast splicing factor Fyv6 ELife 13:RP100449 PMC11651659
White DS, Dunk BM, Vaillancourt FH, and Hoskins AA. 2024. A sequential binding mechanism for 5' splice site recognition and modulation for the human U1 snRNP Nat Commun 15(1):8776 PMC11467380
Love SL, Emerson JD, Koide K, and Hoskins, AA. 2023. Pre-mRNA splicing-associated diseases and therapies RNA Biology 20(1):525-538 PMC10399480
Lipinski KA, Senn KA, Zeps NJ, Hoskins AA. 2023. Biochemical and genetic evidence supports Fyv6 as a second-step splicing factor in Saccharomyces cerevisiae RNA 29(11):1792-1802 PMC10578475
Hansen SR, White DS, Scalf M, Correa Jr IR, Smith LM, Hoskins, AA. 2022. Multi-step recognition of potential 5' splice sites by the Saccharomyces cerevisiae U1 snRNP Elife 11:e70534
Kaur H, van der Feltz C, Sun Y, Hoskins AA. 2022. Network theory reveals principles of spliceosome structure and dynamics Structure 30(1):190-200 PMC8741635
Fu, X, Kaur, H, Rodgers, M L, Montemayor, E J, Butcher, S E, Hoskins, A A. 2022. Identification of transient intermediates during spliceosome activation by single molecule fluorescence microscopy Proc Natl Acad Sci USA 119(48):e2206815119
Lipinski, KA, Chi, J, Chen, X, Hoskins, AA, and Brow DA. 2022. Yeast U6 snRNA made by RNA polymerase II is less stable but functional RNA 28(12):1606-1620 PMC9670810
Carrocci TJ, Hoskins AA. 2021. RNA processing: Fungal spliceosomes break the mold Curre Biol. 31(22):R1482-R1484
Love SL, Hoskins AA. 2021. Stuck on UUUU: New splicing inhibitors enhance U2AF2-RNA binding Cell Chem Biol 28(8):1107-1108
Hoskins AA, Rhoads TW. 2021. When cells are down on their LUC7L2, alternative splicing rewires metabolism for OXPHOS Mol Cell 81(9):1859-1860
van der Feltz C, Nikolai B, Schneider C, Paulson JC, Fu X, and Hoskins AA. 2021. Saccharomyces cerevisiae Ecm2 modulates the catalytic steps of pre-mRNA splicing RNA 27(5):591-603 PMC8051269
Kaur H, Groubert H, Paulson JC, McMillan S, and Hoskins, AA. 2020. Impact of cancer-associated mutations in Hsh155/SF3b1 HEAT repeats 9-12 on pre-mRNA splicing in Saccharomyces cerevisiae PLoS One 15(4):e0229315 PMC7176370
Kaur H, Jamalidinan F, Condon SGF, Senes A, Hoskins AA. 2019. Analysis of spliceosome dynamics by maximum likelihood fitting of dwell time distributions Methods 153:13-21 PMC6363122
Hansen, SR, Nikolai, BJ, Spreacker, PJ, Carrocci, TJ, and Hoskins, AA. . 2019. Chemical Inhibition of Pre-mRNA Splicing in Living Saccharomyces cerevisiae Cell Chem Biol 26(3):443-448 PMC6430687
Beier DH, Carrocci TJ, van der Feltz C, Tretbar US, Paulson JC, Grabowski N, Hoskins AA. 2019. Dynamics of the DEAD-box ATPase Prp5 RecA-like domains provide a conformational switch during spliceosome assembly Nucleic Acids Res 47(20):10842-10851
van der Feltz C and Hoskins AA. 2019. Structural and functional modularity of the U2 snRNP in pre-mRNA splicing Crit Rev Biochem Mol Biol 54(5):2019
van der Feltz, C., DeHaven, A.C., and Hoskins, A.A.. 2018. Stress-induced Pseudouridylation Alters the Structural Equilibrium of Yeast U2 snRNA Stem II J. Mol. Biol. 430(4):524-536 PMC5805610
Carocci, T.J., Paulson, J.C., and Hoskins, A.A.. 2018. Functional analysis of Hsh155/SF3b1 interactions with the U2 snRNA/branch site duplex RNA 24(8):1028-1040 PMC6049509
Condon SGF, Mahbuba DA, Armstrong CR, Diaz-Vazquez G, Craven SJ, LaPointe LM, Khadria AS, Chadda R, Crooks JA, Rangarajan N, Weibel DB, Hoskins AA, Ro. 2018. The FtsLB subcomplex of the bacterial divisome is a tetramer with an uninterrupted FtsL helix linking the transmembrane and periplasmic regions. J Biol Chem 293(5):1623-1641 PMC5798294
Carrocci, T.J., Zoerner, D.M., Paulson, J.C., and Hoskins, A.A.. 2017. SF3b1 mutations associated with myelodysplastic syndromes alter the fidelity of branchsite selection in yeast Nucleic Acids Res. 45(8):4837-4852 PMC5416834
van der Feltz, C. and Hoskins, AA.. 2017. Methodologies for studying the spliceosome's RNA dynamics with single-molecule FRET Methods 125:45-54 PMC5546994
Panchapakesan, SS, Ferguson, ML, Hayden, EJ, Chen, X, Hoskins, AA, and Unrau, PJ. 2017. Ribonucleoprotein Purification and Characterization using RNA Mango RNA :
Larson, JD and Hoskins, AA. 2017. Dynamics and consequences of spliceosome E complex formation Elife :
Didychuk AL, Montemayor EJ, Carrocci TJ, DeLaitsch AT, Lucarelli SE, Westler WM, Brow DA, Hoskins AA, and Butcher SE. 2017. Usb1 controls U6 snRNP assembly through evolutionarily divergent cyclic phosphodiesterase activities Nat Commun 8(1):497 PMC5591277
van der Feltz C, DeHaven AC, and Hoskins AA. 2017. Stress-induced Pseudouridylation Alters the Structural Equilibrium of Yeast U2 snRNA Stem II J Mol Biol. :
Carrocci TJ, Lohe L, Ashton MJ, Hobartner C, and Hoskins AA. 2017. Debranchase-resistant labeling of RNA using the 10DM24 deoxyribozyme and fluorescent modified nucleotides Chem Commun (Cambr). 53(88):11992-11995 PMC5668183
Carrocci TJ and Hoskins AA. 2016. The Spliceosome and pre-mRNA Splicing Encyclopedia of Cell Biology 1:495-502
Xu J., Carrocci TJ., Hoskins AA.. 2016. Evolution and Characterization of a benzylguanine-binding RNA aptamer. Chemical Communications (Cambridge, England) 52(3):549-52 In Process
Rodgers ML., Tretbar SU., DeHaven A., Alwan AA., Luo G., Mast HM., Hoskins AA.. 2016. Conformational Dynamics of Stem II of the U2 snRNA RNA 22(2):225-36 PMC4712673
Cornilescu G., Didychuk AL., Rodgers ML., Michael LA., Burke JE., Montemayor EJ., Hoskins AA., Butcher SE.. 2016. Structural analysis of multi-helical RNAs by NMR-SAXS/WAXS: Application to the U4/U6 di-snRNA. Journal of Molecular Biology 428(5 pt A):777-89 PMC4790120
DeHaven AC., Norden IS., Hoskins AA.. 2016. Lights, camera, action! Capturing the spliceosome and pre-mRNA splicing with single-molecule fluorescence microscopy. Wiley Interdisciplinary Reviews RNA :
Hoskins AA., Rodgers ML., Friedman LJ., Gelles J., Moore MJ.. 2016. Single molecule analysis reveals reversible and irreversible steps during spliceosome activation. eLife 5:e14166 PMC4922858
Rodgers ML., Didychuk A., Brow DA., Butcher SE., Hoskins AA.. 2016. A Multi-step Model for Facilitated Unwinding of the U4/U6 RNA Duplex Nucleic Acids Res. 44(22):10912-10928 PMC5159527
Hansen, S.R., Rodgers, M.L., and Hoskins, A.A.. 2016. Fluorescent Labeling of Proteins in Whole Cell Extracts for Single-Molecule Imaging Methods Enzymol. 581:83-104
Rodgers ML, Paulson J, Hoskins AA. 2015. Rapid isolation and single-molecule analysis of ribonucleoproteins from cell lysate by SNAP-SiMPull RNA 21(5):1031-41 PMC4408783
Kimble J., Bement WM., Chang Q., Cox BL., Drinkwater NR., Course RL., Hoskins AA., Huttenlocher A., Kreeger PK., Lambert PF., Mailick MR., Miyamoto S.. 2015. Strategies from UW-Madison for rescuing biomedical research in the US eLife 4:e09305 PMC4484056
Carrocci TJ and Hoskins AA. 2014. Imaging of RNAs in live cells with spectrally diverse small molecule fluorophores Analyst 139(1):44-7 PMC3873762
Wang, TY, Friedman, LJ, Gelles, J., Min, W., Hoskins, AA, and Cornish, VW.. 2014. The Covalent Trimethoprim Chemical Tag Facilitates Single Molecule Imaging with Organic Fluorophores Biophysical J 106(1):272-8
Larson, JD., Rodgers, ML, and Hoskins, AA. 2014. Visualizing cellular machines with colocalization single molecule spectroscopy Chem Soc Rev 43(4):1189-200 PMC3946777
Trang, VH Rodgers, ML, Boyle, KJ, Hoskins, AA, Strieter, ER. 2014. Chemoenzymatic synthesis of bifunctional polyubiquitin substrates for monitoring ubiquitin chain remodeling ChemBioChem 15(11):1563-8
Larson J, Kirk M, Drier EA, O'Brien W, MacKay JF, Friedman LJ, Hoskins AA. 2014. Design and construction of a multwavelength, micro mirror total internal reflectance fluorescence microscope Nat Protoc 9(10):2317-28
Crawford DJ, Hoskins AA, Friedman LJ, Gelles J, Moore MJ. 2013. Single-molecule colocalization FRET evidence that spliceosome activation precedes stable approach of 5' splice site and branch site. Proc. Natl. Acad. Sci. U.S.A. 110(17):6783-8 (PMC3637735)
Shcherbakova I, Hoskins AA, Friedman LJ, Serebrov V, Corrêa IR, Xu MQ, Gelles J, Moore MJ. 2013. Alternative Spliceosome Assembly Pathways Revealed by Single-Molecule Fluorescence Microscopy. Cell Rep :
Hoskins AA, Moore MJ. 2012. The spliceosome: a flexible, reversible macromolecular machine. Trends Biochem. Sci. 37(5):179-88 (PMC3508674)
Hoskins AA, Gelles J, Moore MJ. 2011. New insights into the spliceosome by single molecule fluorescence microscopy. Curr Opin Chem Biol 15(6):864-70 (PMC3310177)
Hoskins AA, Friedman LJ, Gallagher SS, Crawford DJ, Anderson EG, Wombacher R, Ramirez N, Cornish VW, Gelles J, Moore MJ. 2011. Ordered and dynamic assembly of single spliceosomes. Science 331(6022):1289-95 (PMC3086749)
Morar M, Hoskins AA, Stubbe J, Ealick SE. 2008. Formylglycinamide ribonucleotide amidotransferase from Thermotoga maritima: structural insights into complex formation. Biochemistry 47(30):7816-30 (PMC2646663)
Crawford DJ, Hoskins AA, Friedman LJ, Gelles J, Moore MJ. 2008. Visualizing the splicing of single pre-mRNA molecules in whole cell extract. RNA 14(1):170-9 (PMC2151038)
Hoskins AA, Morar M, Kappock TJ, Mathews II, Zaugg JB, Barder TE, Peng P, Okamoto A, Ealick SE, Stubbe J. 2007. N5-CAIR mutase: role of a CO2 binding site and substrate movement in catalysis. Biochemistry 46(10):2842-55 (PMC2569195)
Morar M, Anand R, Hoskins AA, Stubbe J, Ealick SE. 2006. Complexed structures of formylglycinamide ribonucleotide amidotransferase from Thermotoga maritima describe a novel ATP binding protein superfamily. Biochemistry 45(50):14880-95 (PMC2527724)
Anand R, Hoskins AA, Bennett EM, Sintchak MD, Stubbe J, Ealick SE. 2004. A model for the Bacillus subtilis formylglycinamide ribonucleotide amidotransferase multiprotein complex. Biochemistry 43(32):10343-52
Anand R, Hoskins AA, Stubbe J, Ealick SE. 2004. Domain organization of Salmonella typhimurium formylglycinamide ribonucleotide amidotransferase revealed by X-ray crystallography. Biochemistry 43(32):10328-42
Hoskins AA, Anand R, Ealick SE, Stubbe J. 2004. The formylglycinamide ribonucleotide amidotransferase complex from Bacillus subtilis: metabolite-mediated complex formation. Biochemistry 43(32):10314-27